Description: Modern Applications of DNA Amplification Techniques : Problems and New Tools, Paperback by Lassner, Dirk (EDT); Pustowoit, Barbara (EDT); Rolfs, Arndt (EDT), ISBN 1461374553, ISBN-13 9781461374558, Like New Used, Free shipping in the US In the ten years since the first publication on PCR (Saiki et al. , 1985), this in vitro method of nucleic acid replication and modification has grown to rival in popularity traditional microbiological, genetical und technical procedures for cloning, sequencing, gene detecting and related procedures. To date the PCR literature has emphasized six main areas of application: genetic mapping, detection of mutations, genetic polymorphism, transcriptional splicing and regulation, molecular virology and quantitative procedures. The overwhelming focus of quantification of DNA or RNA by PCR has been on human microbiology and oncological problems. The exquisite sensitivity of PCR gives this method the ability to detect extremely rare DNAs, mRNAs, mRNAs in small numbers of cells or in small amounts of tissue, and mRNAs expressed in mixed-cell populations. However, the exact and accurate quantification of specific nucleic acids in biological samples is in spite of numerous publications in that field still a general problem: during the peR process, an unknown initial number of target sequences are used as a template from which a large quantity of specific product can be obtained. Although the amount of product formed is easy to determine, it is difficult to deduce the initial copy number of the target molecule because the efficiency of the peR is largely unknown.
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Book Title: Modern Applications of DNA Amplification Techniques : Problems an
Number of Pages: VIII, 143 Pages
Publication Name: Modern Applications of D N A Amplification Techniques : Problems and New Tools
Language: English
Publisher: Springer
Publication Year: 2012
Subject: Biotechnology, Life Sciences / Anatomy & Physiology (See Also Life Sciences / Human Anatomy & Physiology), Life Sciences / Zoology / General
Item Weight: 10.9 Oz
Type: Textbook
Author: Barbara Pustowoit
Item Length: 10 in
Subject Area: Science
Item Width: 7 in
Format: Trade Paperback
