Laser Capture Microdissection: Methods and Protocols by Graeme I. Murray (Englis

Description: Laser Capture Microdissection by Graeme I. Murray Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology. Notes Provides practical methods and protocols involving tissue-based laser microdissection, ideal for labs around the globeFully updates and reexamines the popular first editionFeatures key notes and expert implementation advice Back Cover Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols , experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology. Table of Contents Laser Capture Microdissection: Methods and Applications.- Laser Microdissection for Gene Expression Profiling.- Gene Expression Using the PALM System.- Immuno-Guided Microdissection Techniques.- Optimized RNA Extraction from Non-Deparaffinized, Laser-Microdissected Material.- Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue.- MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue.- Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh Frozen Tissue Sections.- Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue.- Detection and Quantification of MicroRNAs in Laser Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues.- Laser Capture Microdissection Applications in Breast Cancer Proteomics.- Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS.- LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues.- Purification of Diseased Cells from Barretts Esophagus and Related Lesions by Laser Capture Microdissection.- Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis.- Application of Laser Microdissection and Quantitative PCR to Assess the Response of Oesophageal Cancer to Neoadjuvant Chemo-Radiotherapy.- Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer.- Evaluation of Gastrointestinal mtDNA Depletion in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE).- Laser Microdissection for Gene Expression Study of Hepatocellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers.- Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to AnalyzeEzH2 by Western Blot Analysis.- Laser-Capture Microdissection of Renal Tubule Cells and Linear Amplification of RNA for Microarray Profiling and Real-Time PCR.- Subcellular Renal Proximal Tubular Mitochondrial Toxicity with Tenofovir Treatment.- Application of Laser Capture Microdissection to Study Renal Carcinogenesis.- Laser Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer.- Quantitative Analysis of the Enzymes Associated with 5-Fluorouracil Metabolism in Prostate Cancer Biopsies.- Microdissection of Gonadal Tissues for Gene Expression Analyses.- Duplex Real Time PCR Assay for Quantifying Mitochondrial DNA Deletions in Laser Microdissected Single Spiral Ganglion Cells.- Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection.- Region-Specific In Situ Hybridization-Guided Laser Capture Microdissection on Postmortem Human Brain Tissue Coupled with Gene Expression Quantification.- UV-Laser Microdissection and mRNA Expression Analysis of Individual Neurons from Post Mortem Parkinsons Disease Brains.- Transcriptome Profiling of Murine Spinal Neurulation Using Laser Capture Microdissection and High-Density Oligonucleotide Microarrays.- Probing the CNS Microvascular Endothelium by Immune-Guided Laser Capture Microdissection Coupled to Quantitative RT-PCR.- Laser Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues.- Laser Capture Microdissection and Analysis of Liver Endothelial Cells from Patients with Budd Chiari Syndrome.- Laser Capture Microdissection of Hyperlipidemic/ApoE-/- Mouse Aorta Atherosclerosis.- Gene Expression Profiling in Laser Microdissected Bone Marrow Megakaryocytes.- Specific RNA Collection from the Rat Endolymphatic Sac by LaserCapture Microdissection (LCM): LCM of Very Small Organ Surrounded by Bony Tissue.- The Use of Laser Capture Microdissection on Adult Human Articular Cartilage for Gene Expression Analysis.- Laser Capture Microdissection of Developing Barley Seeds and cDNA Array Analysis of Selected Tissues.- Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia. Feature Provides practical methods and protocols involving tissue-based laser microdissection, ideal for labs around the globe Fully updates and reexamines the popular first edition Features key notes and expert implementation advice Details ISBN1617791628 Publisher Humana Press Inc. Series Methods in Molecular Biology Year 2011 Edition 2nd ISBN-10 1617791628 ISBN-13 9781617791628 Format Hardcover Imprint Humana Press Inc. Place of Publication Totowa, NJ Country of Publication United States Edited by Graeme I. Murray Short Title LASER CAPTURE MICRODISSECTION Language English Media Book Series Number 755 Subtitle Methods and Protocols Replaces 9781588292605 Publication Date 2011-07-19 Pages 493 DEWEY 572.8 DOI 10.1007/978-1-61779-163-5 AU Release Date 2011-07-19 NZ Release Date 2011-07-19 US Release Date 2011-07-19 UK Release Date 2011-07-19 Author Graeme I. Murray Edition Description 2nd ed. 2011 Alternative 9781493960804 Audience Professional & Vocational Illustrations 1 Illustrations, color; 96 Illustrations, black and white; XVII, 493 p. 97 illus., 1 illus. in color. We've got this At The Nile, if you're looking for it, we've got it. With fast shipping, low prices, friendly service and well over a million items - you're bound to find what you want, at a price you'll love! TheNile_Item_ID:96370156;

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